Journal: bioRxiv
Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth
doi: 10.1101/2025.07.09.663961
Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.
Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay